{"id":87423,"date":"2023-08-01T11:08:31","date_gmt":"2023-08-01T03:08:31","guid":{"rendered":"http:\/\/www.broadbio.com\/?p=87423"},"modified":"2023-08-01T11:08:37","modified_gmt":"2023-08-01T03:08:37","slug":"%e5%8a%a0%e6%8b%bf%e5%a4%a7nrc%e5%86%88%e7%94%b0%e8%bd%af%e6%b5%b7%e7%bb%b5%e9%85%b8%e8%ae%a4%e8%af%81%e6%a0%a1%e5%87%86%e6%ba%b6%e6%b6%b2%e6%a0%87%e5%87%86%e5%93%81crm-oa%e5%88%b6%e5%a4%87","status":"publish","type":"post","link":"http:\/\/www.broadbio.com\/archives\/87423","title":{"rendered":"\u52a0\u62ff\u5927NRC\u5188\u7530\u8f6f\u6d77\u7ef5\u9178\u8ba4\u8bc1\u6821\u51c6\u6eb6\u6db2\u6807\u51c6\u54c1CRM-OA\u5236\u5907"},"content":{"rendered":"\n

\u5188\u7530\u8f6f\u6d77\u7ef5\u9178\u8ba4\u8bc1\u6821\u51c6\u6eb6\u6db2<\/p>\n\n\n\n

\u52a0\u62ff\u5927NRC\u5188\u7530\u8f6f\u6d77\u7ef5\u9178\u8ba4\u8bc1\u6821\u51c6\u6eb6\u6db2\u6807\u51c6\u54c1CRM-OA<\/strong>\u5188\u7530\u8f6f\u6d77\u7ef5\u9178\uff08OA\uff09\u662f\u4e00\u79cd\u7531\u6d77\u6d0b\u7532\u85fb\u4ea7\u751f\u7684\u6bd2\u7d20\uff0c\u4e0e\u8179\u6cfb\u6027\u8d1d\u7c7b\u4e2d\u6bd2\uff08DSP\uff09\u6709\u5173\uff0c\u8fd9\u662f\u4e00\u79cd\u56e0\u98df\u7528\u53d7\u6c61\u67d3\u7684\u8d1d\u7c7b\u800c\u5f15\u8d77\u7684\u80c3\u80a0\u9053\u7efc\u5408\u5f81\u3002\u8d1d\u7c7b\u4e2dDSP\uff08OA\u3001DTX1\u548cDTX2\uff09\u7684\u6700\u5927\u5141\u8bb8\u603b\u6c34\u5e73\u76ee\u524d\u4e3a\u6574\u4e2a\u7ec4\u7ec7\u4e2d0.16 mg OA\u5f53\u91cf\/kg[1]\u3002CRM-OA-d\u662f\u4e00\u79cd\u7ecf\u8ba4\u8bc1\u7684OA\u7532\u9187\u6821\u51c6\u6eb6\u6db2\uff0c\u662f2007\u5e74\u53d1\u5e03\u7684CRM-OA-c\u7684\u66ff\u4ee3\u54c1\u3002<\/p>\n\n\n\n

Okadaic acid    \u5188\u7530\u8f6f\u6d77\u7ef5\u9178<\/p>\n\n\n\n

CAS registry No.: 78111-17-8          CAS\u6ce8\u518c\u53f7\uff1a78111-17-8<\/p>\n\n\n\n

Molecular formula: C44H68O13    \u5206\u5b50\u5f0f\uff1aC44H68O13<\/p>\n\n\n\n

Molecular weight: 805.00 g\/mol    \u5206\u5b50\u91cf\uff1a805.00\ng\/mol<\/p>\n\n\n\n

[M+H]+: m\/z 805.4733    [M+H]+\uff1aM\/z 805.4733<\/p>\n\n\n\n

[M-H]-: m\/z 803.4587      [M-H]-\uff1aM\/z 803.4587<\/p>\n\n\n\n

\u9884\u671f\u7528\u9014<\/strong><\/p>\n\n\n\n

\u52a0\u62ff\u5927NRC\u5188\u7530\u8f6f\u6d77\u7ef5\u9178\u8ba4\u8bc1\u6821\u51c6\u6eb6\u6db2\u6807\u51c6\u54c1CRM-OA<\/strong> CRM-OA-d\u662f\u4e00\u79cd\u7ecf\u8fc7\u8ba4\u8bc1\u7684\u6821\u51c6\u6eb6\u6db2\uff0c\u65e8\u5728\u5f00\u53d1\u5206\u6790\u65b9\u6cd5\u548c\u51c6\u786e\u5b9a\u91cfOA\u3002CRM-OA-d\u7684\u6d53\u5ea6\u4f7f\u5176\u9002\u5408\u4e8e\u5236\u5907\u7528\u4e8e\u6821\u51c6\u6db2\u76f8\u8272\u8c31\uff08LC\uff09\u548c\u6db2\u76f8\u8272\u8c31-\u8d28\u8c31\uff08LC-MS\uff09\u4eea\u5668\u7684\u7a00\u91ca\u7cfb\u5217\uff0c\u4ee5\u53ca\u7528\u4e8e\u56de\u6536\u5b9e\u9a8c\u7684\u8d1d\u7c7b\u5bf9\u7167\u6837\u54c1\u7684\u52a0\u6807\u3002<\/p>\n\n\n\n

\u50a8\u5b58\u548c\u4f7f\u7528\u8bf4\u660e<\/strong><\/p>\n\n\n\n

\u52a0\u62ff\u5927NRC\u5188\u7530\u8f6f\u6d77\u7ef5\u9178\u8ba4\u8bc1\u6821\u51c6\u6eb6\u6db2\u6807\u51c6\u54c1CRM-OA<\/strong>\u4e3a\u786e\u4fddCRM-OA-d\u7684\u7a33\u5b9a\u6027\uff0c\u5b89\u74ff\u5e94\u50a8\u5b58\u5728-12\u00b0C\u6216\u4ee5\u4e0b\u3002\u6253\u5f00\u524d\uff0c\u5e94\u5c06\u6bcf\u4e2a\u5b89\u74ff\u52a0\u70ed\u81f3\u5ba4\u6e29\uff0c\u5e76\u5c06\u5185\u5bb9\u7269\u5145\u5206\u6df7\u5408\u3002\u5b89\u74ff\u5e94\u5728\u9884\u5148\u523b\u597d\u7684\u6807\u8bb0\u5904\u6253\u5f00\u3002\u5b89\u74ff\u6253\u5f00\u540e\uff0c\u5e94\u4f7f\u7528\u6821\u51c6\u7684\u5bb9\u91cf\u8bbe\u5907\u53d6\u51fa\u51c6\u786e\u7684\u7b49\u5206\u8bd5\u6837\uff0c\u5e76\u5c06\u5176\u8f6c\u79fb\u5230\u5bb9\u91cf\u74f6\u6216\u5c0f\u74f6\u4e2d\u3002\u5982\u679c\u6eb6\u6db2\u6253\u5f00\u8d85\u8fc7\u51e0\u5206\u949f\uff0c\u5219\u4f1a\u56e0\u7532\u9187\u84b8\u53d1\u800c\u5bfc\u81f4\u6d53\u5ea6\u589e\u52a0\u3002\u5efa\u8baeCRM-OA-d\u6eb6\u6db2\u4e0d\u5e94\u84b8\u53d1\u81f3\u5e72\uff0c\u56e0\u4e3a\u73bb\u7483\u8868\u9762\u53ef\u80fd\u4f1a\u6709\u635f\u5931\u3002\u6ce8\uff1a\u6eb6\u6db2\u7684\u4f53\u79ef\u672a\u7ecf\u8ba4\u8bc1\uff1b\u53ea\u6709\u6d53\u5ea6\u88ab\u8bc1\u660e\u3002\u56e0\u6b64\uff0c\u5b89\u74ff\u7684\u5168\u90e8\u5185\u5bb9\u7269\u4e0d\u5e94\u7b80\u5355\u5730\u8f6c\u79fb\u5230\u5bb9\u91cf\u74f6\u4e2d\u5e76\u7a00\u91ca\u81f3\u4e00\u5b9a\u4f53\u79ef\u3002<\/p>\n\n\n\n

CRM-OA-d<\/strong>\u7684\u5236\u5907<\/strong><\/p>\n\n\n\n

\u52a0\u62ff\u5927NRC\u5188\u7530\u8f6f\u6d77\u7ef5\u9178\u8ba4\u8bc1\u6821\u51c6\u6eb6\u6db2\u6807\u51c6\u54c1CRM-OA<\/strong> OA\u662f\u4ece\u7532\u85fb\u7269\u79cd\u51f9\u539f\u7684\u5b9e\u9a8c\u5ba4\u57f9\u517b\u7269\u4e2d\u5206\u79bb\u51fa\u6765\u7684\uff0c\u5e76\u5728\u54c8\u5229\u6cd5\u514b\u65af\u56fd\u5bb6\u7814\u7a76\u59d4\u5458\u4f1a\u901a\u8fc7\u51e0\u4e2a\u8272\u8c31\u6b65\u9aa4\u548c\u7ed3\u6676\u8fdb\u884c\u7eaf\u5316\u3002\u901a\u8fc71H NMR\u548cLC-MS\/MS\u786e\u8bc1\u4e86OA\u7684\u7ed3\u6784\u548c\u7eaf\u5ea6\u3002\u4f7f\u7528\u9ad8\u5206\u8fa8\u7387MS\u83b7\u5f97OA[m-H]-\u7684\u6d4b\u91cf\u51c6\u786em\/z\u4e3a803.4587\u00b10.0001\uff08\u5bf9\u4e8eC44H67O13-\uff0c\u2206=-0.1 ppm\uff09\uff08\u56fe1A\uff09\u3002\u78b0\u649e\u8bf1\u5bfc\u7684\u79bb\u89e3\u5149\u8c31\uff08\u56fe1B\uff09\u4e0e\u4ee5\u524d\u7684OA CRM\u548c\u6587\u732e\u62a5\u9053[2]\u76f8\u5339\u914d\u3002<\/p>\n\n\n\n

\u901a\u8fc7\u5c06OA\u6eb6\u89e3\u5728CD3OH\u4e2d\u7528\u4e8e\u5b9a\u91cfNMR\uff08qNMR\uff09\u6765\u5236\u5907\u50a8\u5907\u6eb6\u6db2[3]\u3002CRM\u6eb6\u6db2\u662f\u901a\u8fc7\u5728\u8131\u6c14\u7684\u9ad8\u7eaf\u5ea6\u7532\u9187\u4e2d\u7cbe\u786e\u7a00\u91caqNMR\u50a8\u5907\u6eb6\u6db2\u6765\u5236\u5907\u7684\u3002\u5c06\u8be5\u6eb6\u6db2\u7528Teflon\u6405\u62cc\u68d2\u548c\u78c1\u529b\u6405\u62cc\u5668\u5145\u5206\u6df7\u5408\uff0c\u540c\u65f6\u5728\u6c29\u6c14\u6c1b\u4e0b\u7684\u51b0\u6d74\u4e2d\u51b7\u5374\u3002\u5c06\u7b49\u5206\u8bd5\u6837\u5206\u914d\u5230\u5145\u6ee1\u6c29\u6c14\u7684\u7425\u73c0\u8272\u73bb\u7483\u5b89\u74ff\u4e2d\uff0c\u5e76\u7acb\u5373\u8fdb\u884c\u706b\u7130\u5bc6\u5c01\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u5188\u7530\u8f6f\u6d77\u7ef5\u9178\u8ba4\u8bc1\u6821\u51c6\u6eb6\u6db2 \u52a0\u62ff\u5927NRC\u5188\u7530\u8f6f\u6d77\u7ef5\u9178\u8ba4\u8bc1\u6821\u51c6\u6eb6\u6db2\u6807\u51c6\u54c1CRM-OA\u5188\u7530\u8f6f\u6d77\u7ef5\u9178\uff08OA\uff09\u662f\u4e00\u79cd\u7531\u6d77 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[92],"tags":[],"_links":{"self":[{"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/posts\/87423"}],"collection":[{"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/comments?post=87423"}],"version-history":[{"count":1,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/posts\/87423\/revisions"}],"predecessor-version":[{"id":87429,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/posts\/87423\/revisions\/87429"}],"wp:attachment":[{"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/media?parent=87423"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/categories?post=87423"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/tags?post=87423"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}