{"id":104257,"date":"2025-09-16T09:25:31","date_gmt":"2025-09-16T01:25:31","guid":{"rendered":"http:\/\/www.broadbio.com\/?post_type=product&p=104257"},"modified":"2025-09-16T09:25:53","modified_gmt":"2025-09-16T01:25:53","slug":"nist-srm-1955%e5%86%b7%e5%86%bb%e4%ba%ba%e8%a1%80%e6%b8%85%e4%b8%ad%e5%90%8c%e5%9e%8b%e5%8d%8a%e8%83%b1%e6%b0%a8%e9%85%b8%e5%92%8c%e5%8f%b6%e9%85%b8%e6%a0%87%e5%87%86%e5%93%81","status":"publish","type":"product","link":"http:\/\/www.broadbio.com\/product\/nist-srm-1955%e5%86%b7%e5%86%bb%e4%ba%ba%e8%a1%80%e6%b8%85%e4%b8%ad%e5%90%8c%e5%9e%8b%e5%8d%8a%e8%83%b1%e6%b0%a8%e9%85%b8%e5%92%8c%e5%8f%b6%e9%85%b8%e6%a0%87%e5%87%86%e5%93%81","title":{"rendered":"NIST SRM 1955\u51b7\u51bb\u4eba\u8840\u6e05\u4e2d\u540c\u578b\u534a\u80f1\u6c28\u9178\u548c\u53f6\u9178\u6807\u51c6\u54c1"},"content":{"rendered":"\n\n\n\n\n\n\n\n\n\n\n\n
\u4ea7\u54c1\u8d27\u53f7<\/td>\nSRM 1955\/NIST SRM 1955<\/strong><\/td>\n<\/tr>\n
\u4ea7\u54c1\u540d\u79f0<\/td>\nHomocysteine and Folate in Frozen Human Serum<\/strong><\/td>\n<\/tr>\n
\u4e2d\u6587\u540d\u79f0<\/td>\n\u51b7\u51bb\u4eba\u8840\u6e05\u4e2d\u7684\u540c\u578b\u534a\u80f1\u6c28\u9178\u548c\u53f6\u9178\u6807\u51c6\u53c2\u8003\u7269\u8d28<\/strong>\/<\/strong>\u51b7\u51bb\u4eba\u8840\u6e05\u4e2d\u540c\u578b\u534a\u80f1\u6c28\u9178\u548c\u53f6\u9178<\/strong>\u6807\u51c6\u7269\u8d28\/<\/strong>\u51b7\u51bb\u4eba\u8840\u6e05\u4e2d\u540c\u578b\u534a\u80f1\u6c28\u9178\u548c\u53f6\u9178<\/strong>\u6807\u51c6\u54c1<\/strong><\/td>\n<\/tr>\n
\u4ea7\u54c1\u89c4\u683c<\/td>\nset (3) (1 each conc)<\/td>\n<\/tr>\n
\u4ea7\u54c1\u8be6\u60c5<\/td>\n\u4e3b\u8981\u7528\u4e8e\u8bc4\u4f30\u6d4b\u5b9a\u4eba\u4f53\u8840\u6e05\u4e2d\u540c\u578b\u534a\u80f1\u6c28\u9178\u548c\u53f6\u9178\uff08\u5404\u79cd\u5f62\u5f0f\uff09\u7684\u4e34\u5e8a\u7a0b\u5e8f\u7684\u51c6\u786e\u6027\u3002\u5b83\u4e5f\u7528\u4e8e\u9a8c\u8bc1\u5de5\u4f5c\u6216\u6b21\u8981\u53c2\u8003\u6750\u6599<\/td>\n<\/tr>\n
\u4ea7\u54c1\u6709\u6548\u671f<\/td>\n31-Dec-18<\/td>\n<\/tr>\n
\u8bc1\u4e66\u66f4\u65b0\u65f6\u95f4<\/td>\n31-Dec-15<\/td>\n<\/tr>\n
\u50a8\u5b58\u6e29\u5ea6<\/td>\n\u51b7\u51bb-80\u6444\u6c0f\u5ea6\/\u6709\u5173\u6b64\u6750\u6599\u7684\u5b58\u50a8\u4fe1\u606f\uff0c\u8bf7\u53c2\u9605\u8bc1\u4e66\u3002<\/td>\n<\/tr>\n
\u5371\u9669\u7b49\u7ea7<\/td>\n\u975e\u5371<\/td>\n<\/tr>\n
\u6613\u8150\u7c7b\u578b<\/td>\n\u8fd9\u79cd\u6750\u6599\u6613\u8150\u70c2\uff0c\u5e72\u51b0\u8fd0\u8f93<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

NIST SRM 1955\u7684\u6298\u53e0\u6d4b\u91cf<\/strong>\uff1a<\/strong>\u5bf9\u4e8eNIST\u76845MT\u6d4b\u91cf\uff0c\u4f7f\u7528\u4e86\u4e09\u79cd\u57fa\u4e8eLC\/MS\/MS\u7684\u65b9\u6cd5\u53d8\u4f53[3\uff0c4\uff0c9]\u3002<\/p>\n

\u51b7\u51bb\u4eba\u8840\u6e05\u4e2d\u540c\u578b\u534a\u80f1\u6c28\u9178\u548c\u53f6\u9178\u6807\u51c6\u54c1\u65b9\u6cd51<\/strong>\uff1a\u5bf9\u4e8e\u6bcf\u4e2a\u6837\u54c1\uff0c\u5728500\u03bcL SRM 1955\u7b49\u5206\u8bd5\u6837\u4e2d\u52a0\u5165[13C5]-5MT\u50a8\u5907\u6807\u51c6\uff0c\u7acb\u5373\u7528\u7b49\u4f53\u79ef\u7684\u542b\u6709\uff08\u8d28\u91cf\u6d53\u5ea6\uff0910%\u6297\u574f\u8840\u9178\u548c1%\u4e59\u4e8c\u80fa\u56db\u4e59\u9178\uff08EDTA\uff09\u7684\u6c34\u6eb6\u6db2\u7a00\u91ca\uff0c\u5e76\u5728\u51b0\u4e0a\u5e73\u8861\u226515\u5206\u949f\u3002\u4f7f\u7528C18 SPE\u67f1\u4ece\u6837\u54c1\u4e2d\u63d0\u53d65MT\uff1a\u7532\u9187\u3001\u6c34\u548c1%\u6297\u574f\u8840\u9178\/1%EDTA\u6eb6\u6db2\u7528\u4e8e\u8c03\u8282\uff0c95+5\u6c34+\u7532\u9187\u6eb6\u6db2\u7528\u4e8e\u6d17\u6da4\uff0c1%\u6297\u574f\u8840\u9178\/1%\u7532\u9178\u572850+50\u6c34+\u7532\u9187\u4e2d\u7528\u4e8e\u5206\u6790\u7269\u6d17\u8131\u3002\u4f7f\u7528\u5177\u6709\u7535\u55b7\u96fe\u7535\u79bb\u6e90\u548c\u4e09\u91cd\u56db\u6781\u8d28\u91cf\u5206\u6790\u4eea\u7684\u5546\u7528LC\/MS\/MS\u4eea\u5668\u8fdb\u884c\u63d0\u53d6\u7269\u5206\u6790\u3002\u4f7f\u7528\u7532\u9178\u5728\u6c34\u548c\u7532\u9187\u4e2d\u7684\u68af\u5ea6\u5728\u82ef\u57fa\u4e19\u57faLC\u67f1\uff083.9 mm\u00d7150 mm\uff0c4\u03bcm\u7c92\u5f84\uff09\u4e0a\u8fdb\u884cLC\u5206\u79bb\u3002\u5728\u591a\u91cd\u53cd\u5e94\u76d1\u6d4b\uff08MRM\uff09\u6a21\u5f0f\u4e0b\u5bf95MT\u548c[13C5]-5MT\u8fdb\u884cLC\/MS\/MS\u68c0\u6d4b\u548c\u5b9a\u91cf\u3002\u9488\u5bf9\u8d28\u5b50\u5316\u7684\u5206\u6790\u7269\u5206\u5b50[M+H]+\u548c\u7a33\u5b9a\u7684\u8d28\u5b50\u5316\u7247\u6bb5\uff0c\u5bf9\u6bcf\u79cd\u5206\u6790\u7269\u7684MRM\u8f6c\u53d8\u8fdb\u884c\u4e86\u5355\u72ec\u4f18\u5316\u3002\u5bf9\u4e8e5MT\uff0c\u76f8\u5173\u7684MRM\u8d28\u91cf\u8f6c\u53d8\u4e3am\/z 460\u2192m\/z 313\uff0c\u800c\u5bf9\u4e8e[135]-5MT\uff0c\u5219\u4e3am\/z 465\u2192m\/z 313\u3002\u901a\u8fc7\u7ebf\u6027\u63d2\u503c\u4ece\u72ec\u7acb\u6784\u5efa\u7684\u6bcf\u7ec4\u6837\u54c1\u7684\u6821\u51c6\u66f2\u7ebf\u8ba1\u7b97\u5206\u6790\u7269\u6d53\u5ea6\u3002<\/p>\n

\u51b7\u51bb\u4eba\u8840\u6e05\u4e2d\u540c\u578b\u534a\u80f1\u6c28\u9178\u548c\u53f6\u9178<\/strong>\u6807\u51c6\u54c1\u65b9\u6cd52<\/strong>\uff1a\u6309\u7167\u65b9\u6cd51\uff0c\u5bf9\u8bd5\u9a8c\u90e8\u5206\u8fdb\u884c\u52a0\u6807\u548c\u5e73\u8861\u3002\u7528\u504f\u78f7\u9178\u6c89\u6dc0\u86cb\u767d\u8d28\uff0c\u5e76\u901a\u8fc7\u52a0\u5165\u542b\u67090.4mol\/L K2HPO4\u548c0.5mol\/L NaOH\u7684\u6eb6\u6db2\u6765\u4e2d\u548c\u5269\u4f59\u7684\u4e0a\u6e05\u6db2\u3002\u4f7f\u7528\u56fa\u76f8\u4eb2\u548c\u8403\u53d6\uff08SPAE\uff09\u67f1\u4ece\u6837\u54c1\u4e2d\u63d0\u53d65MT\uff1a1%\u6297\u574f\u8840\u9178\uff08\u8d28\u91cf\u6d53\u5ea6\uff09\u548c0.1mol\/L\u7518\u6c28\u9178-HCl\u6eb6\u6db2\uff08pH 3.0\uff09\uff0c\u6c34\u548c50mmol\/L\u78f7\u9178\u94be\u7f13\u51b2\u6db2\uff08pH 7.4\uff09\u7528\u4e8e\u8c03\u8282\uff0c\u6c34\u7528\u4e8e\u6d17\u6da4\uff0c\u6297\u574f\u8840\u9178\/\u7518\u6c28\u9178-HCl\u6db2\u7528\u4e8e\u5206\u6790\u7269\u6d17\u8131\u3002\u6309\u7167\u65b9\u6cd51\u8fdb\u884cLC\/MS\/MS\u3002<\/p>\n

\u51b7\u51bb\u4eba\u8840\u6e05\u4e2d\u540c\u578b\u534a\u80f1\u6c28\u9178\u548c\u53f6\u9178<\/strong>\u6807\u51c6\u7269\u8d28\u65b9\u6cd53<\/strong>\uff1a\u5bf9\u4e8e5MT\u548cFA\u7684\u5206\u6790\uff0c\u6837\u54c1\u5236\u5907\u548c\u4eea\u5668\u4e0e\u65b9\u6cd51\u6240\u8ff0\u76f8\u540c\u3002\u5728C18 LC\u67f1\uff084.6mm\u00d7150mm\uff0c\u7c92\u5f845\u03bcm\uff09\u4e0a\uff0c\u4f7f\u7528\u7532\u9178\u5728\u6c34\u548c\u7532\u9187\u4e2d\u7684\u68af\u5ea6\u8fdb\u884cLC\u5206\u79bb\u3002\u5728\u591a\u91cd\u53cd\u5e94\u76d1\u6d4b\uff08MRM\uff09\u6a21\u5f0f\u4e0b\u5bf95MT\/[13C5]-5MT\u548cFA\/[13C5]-FA\u8fdb\u884cLC\/MS\/MS\u68c0\u6d4b\u548c\u5b9a\u91cf\u3002\u9488\u5bf9\u8d28\u5b50\u5316\u7684\u5206\u6790\u7269\u5206\u5b50[M+H]+\u548c\u7a33\u5b9a\u7684\u8d28\u5b50\u5316\u7247\u6bb5\uff0c\u5bf9\u6bcf\u79cd\u5206\u6790\u7269\u7684MRM\u8f6c\u53d8\u8fdb\u884c\u4e86\u5355\u72ec\u4f18\u5316\u3002MRM\u8d28\u91cf\u8f6c\u53d8\uff1a\u5bf9\u4e8e5MT\uff0cm\/z 460\u2192m\/z 313\uff0c\u5bf9\u4e8e[13C5]-5MT\uff0cm\/z 465\u2192m\/z 313FA\uff0cm\/z 442\u2192m\/z295 FA\uff0c\u4ee5\u53ca\u5bf9\u4e8e[13C5]-FA\uff0cm\/z 445\u2192m\/z 295\u3002\u901a\u8fc7\u5bf9\u6bcf\u7ec4\u6837\u54c1\u72ec\u7acb\u6784\u5efa\u7684\u6821\u51c6\u66f2\u7ebf\u8fdb\u884c\u7ebf\u6027\u63d2\u503c\u8ba1\u7b97\u5206\u6790\u7269\u6d53\u5ea6\u3002<\/p>\n

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