{"id":103880,"date":"2025-08-20T09:15:23","date_gmt":"2025-08-20T01:15:23","guid":{"rendered":"http:\/\/www.broadbio.com\/?post_type=product&p=103880"},"modified":"2025-08-20T09:15:29","modified_gmt":"2025-08-20T01:15:29","slug":"nist-srm-998%e4%ba%ba%e8%a1%80%e7%ae%a1%e7%b4%a7%e5%bc%a0%e7%b4%a0i%e6%a0%87%e5%87%86%e7%89%a9%e8%b4%a8","status":"publish","type":"product","link":"http:\/\/www.broadbio.com\/product\/nist-srm-998%e4%ba%ba%e8%a1%80%e7%ae%a1%e7%b4%a7%e5%bc%a0%e7%b4%a0i%e6%a0%87%e5%87%86%e7%89%a9%e8%b4%a8","title":{"rendered":"NIST SRM 998\u4eba\u8840\u7ba1\u7d27\u5f20\u7d20I\u6807\u51c6\u7269\u8d28"},"content":{"rendered":"\n\n\n\n\n\n\n\n\n\n\n\n
\u4ea7\u54c1\u8d27\u53f7<\/td>\nSRM 998\/ NIST SRM 998<\/strong><\/td>\n<\/tr>\n
\u4ea7\u54c1\u540d\u79f0<\/td>\nAngiotensin I (Human)<\/strong><\/td>\n<\/tr>\n
\u4e2d\u6587\u540d\u79f0<\/td>\n\u8840\u7ba1\u7d27\u5f20\u7d20<\/strong>I<\/strong>\uff08\u4eba\uff09<\/strong>\/<\/strong>\u4eba\u8840\u7ba1\u7d27\u5f20\u7d20<\/strong>I\/<\/strong>\u4eba\u8840\u7ba1\u7d27\u5f20\u7d20<\/strong>I<\/strong>\u6807\u51c6\u7269\u8d28<\/strong>\/\u4eba\u8840\u7ba1\u7d27\u5f20\u7d20<\/strong>I<\/strong>\u6807\u51c6\u54c1<\/strong><\/td>\n<\/tr>\n
\u4ea7\u54c1\u89c4\u683c<\/td>\n0.5 mg<\/td>\n<\/tr>\n
\u4ea7\u54c1\u8be6\u60c5<\/td>\n\u672c\u6807\u51c6\u53c2\u8003\u7269\u8d28\uff08SRM\uff09\u4e3b\u8981\u7528\u4e8e\u80be\u7d20\u6d4b\u5b9a\u7684\u6821\u51c6\u548c\u6807\u51c6\u5316\uff0c\u5e76\u4f5c\u4e3a\u6c28\u57fa\u9178\u5206\u6790\u548c\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff08HPLC\uff09\u7684\u53c2\u8003\u80bd\u3002SRM 998\u7684\u4e00\u4e2a\u5355\u4f4d\u75310.5mg\u5316\u5b66\u5408\u6210\u7684\u8840\u7ba1\u7d27\u5f20\u7d20I\u7ec4\u6210\u3002<\/td>\n<\/tr>\n
\u4ea7\u54c1\u6709\u6548\u671f<\/td>\n\u81ea\u88c5\u8fd0\u4e4b\u65e5\u8d775\u5e74<\/td>\n<\/tr>\n
\u8bc1\u4e66\u66f4\u65b0\u65f6\u95f4<\/td>\n19-May-21<\/td>\n<\/tr>\n
\u50a8\u5b58\u6e29\u5ea6<\/td>\n\u51b7\u51bb-20\u6444\u6c0f\u5ea6\/\u6709\u5173\u6b64\u6750\u6599\u7684\u5b58\u50a8\u4fe1\u606f\uff0c\u8bf7\u53c2\u9605\u8bc1\u4e66\u3002<\/td>\n<\/tr>\n
\u5371\u9669\u7b49\u7ea7<\/td>\n\u975e\u5371<\/td>\n<\/tr>\n
\u6613\u8150\u7c7b\u578b<\/td>\n\u8fd9\u79cd\u6750\u6599\u6613\u8150\u70c2\uff0c\u5e72\u51b0\u8fd0\u8f93<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4eba\u8840\u7ba1\u7d27\u5f20\u7d20I\u6807\u51c6\u7269\u8d28\u6765\u6e90\u3001\u5236\u5907\u548c\u5206\u6790\uff08<\/strong>1<\/strong>\uff09<\/strong><\/p>\n

NIST SRM 998<\/strong>\u8be5\u6750\u6599\u4eceBeckman Instruments\uff0c\u80a1\u4efd\u6709\u9650\u516c\u53f8\uff0cBioproducts Operations\uff08Palo Alto\uff0cCA\uff09\u83b7\u5f97\u3002<\/p>\n

\u4f7f\u7528\u57285000 kPa\u538b\u529b\u4e0b\u64cd\u4f5c\u768425 cm x 0.4 cm\u5341\u516b\u70f7\u57fa\u7845\u70f7\u67f1\uff0c\u5bf9\u6eb6\u89e3\u768425\u00b5g\u8be5SRM\u6837\u54c1\u8fdb\u884c\u6db2\u76f8\u8272\u8c31\u3002\u5c06\u6837\u54c1\u6eb6\u89e3\u572881\u4f53\u79ef\u4efd0.1mol\/L pH\u4e3a3.5\u7684\u78f7\u9178\u4e09\u4e59\u80fa\u7f13\u51b2\u6db2\u548c19\u4f53\u79ef\u4efd\u4e59\u8148\u7684\u6eb6\u6db2\u4e2d\u3002\u4f7f\u7528\u4e86\u4e24\u79cd\u6d17\u8131\u6db2\u6eb6\u6db2\uff1a\u4e00\u79cd\u662f\u7528\u4e8e\u6eb6\u89e3SRM\u7684\u76f8\u540c\u7ec4\u6210\u7684\u6eb6\u6db2\uff1b\u53e6\u4e00\u79cd\u662f75\u4f53\u79ef\u4efd\u7f13\u51b2\u6db2\u4e0e25\u4f53\u79ef\u4efd\u4e59\u8148\u7684\u6eb6\u6db2\u3002\u901a\u8fc7\u5728215nm\u548c280nm\u5904\u6d4b\u91cf\u5438\u5149\u5ea6\u6765\u76d1\u6d4b\u6d17\u8131\u3002\u572881\u223619\u7684\u6d17\u8131\u6db2\u6bd4\u4e0b\uff0c\u5728215nm\u5904\u68c0\u6d4b\u5230\u4fdd\u7559\u65f6\u95f4\u5206\u522b\u4e3a16.6\u5206\u949f\u548c12.2\u5206\u949f\u7684\u4e3b\u5cf0\u548c\u6b21\u5cf0\uff0c\u5e76\u4e14\u5728280nm\u5904\u4ec5\u68c0\u6d4b\u5230\u4e3b\u5cf0\u3002\u6536\u96c6\u5bf9\u5e94\u4e8e\u6bcf\u4e2a\u5cf0\u7684\u6750\u6599\uff0c\u57286mol\/L HCl\u4e2d\u6c34\u89e3\u5e76\u5206\u6790\u6c28\u57fa\u9178\u7ec4\u6210\u3002\u4e3b\u5cf0\u7684\u6c28\u57fa\u9178\u542b\u91cf\u8868\u660e\u5b83\u662f\u8840\u7ba1\u7d27\u5f20\u7d20I\u3002\u6b21\u5cf0\uff08\u5728215nm\u5904\u5438\u5149\u5ea6\u76841%\uff09\u4e0d\u5305\u542b\u53ef\u68c0\u6d4b\u7684\u80bd\u7269\u8d28\u3002\u5728\u8fd9\u4e9bHPLC\u6761\u4ef6\u4e0b\uff0c\u4e59\u9178\u76d0\u5728\u6eb6\u5242\u524d\u6cbf\u6d17\u8131\uff0c\u4e0d\u80fd\u5b9a\u91cf\u3002\u572875:25\u7684\u6d17\u8131\u6db2\u6bd4\u4f8b\u4e0b\uff0c\u5728\u4efb\u4e00\u6ce2\u957f\u90fd\u6ca1\u6709\u68c0\u6d4b\u5230\u989d\u5916\u7684\u5cf0\uff0c\u8fd9\u8868\u660e\u4e0d\u5b58\u5728\u975e\u80bd\u7684\u7d2b\u5916\u7ebf\u5438\u6536\u6750\u6599\u3002<\/p>\n

\u901a\u8fc7\u4f7f\u7528400MHz NMR\u5149\u8c31\u4eea\u5728\u8109\u51b2\u5085\u7acb\u53f6\u53d8\u6362\u6a21\u5f0f\u4e0b\u6d4b\u5b9aSRM\u7684\u516d\u4e2a\u6837\u54c1\u7684\u4e59\u9178\u76d0\u542b\u91cf\u3002\u901a\u8fc7\u5c06\u6574\u4e2a\u6837\u54c1\uff08\u7ea60.5mg\uff09\u6eb6\u89e3\u57280.5mL\u6c27\u5316\u6c18\uff08100\u539f\u5b50%D\uff09\u4e2d\u6765\u5236\u5907\u7528\u4e8e\u8be5\u5206\u6790\u7684\u6eb6\u6db2\u3002\u5411\u6bcf\u79cd\u6eb6\u6db2\u4e2d\u52a0\u5165\u5c11\u91cf4,4-\u4e8c\u7532\u57fa-4-\u7845\u620a\u9178\u94a02,2,3,3-d4\u4f5c\u4e3a\u5185\u6807\u3002\u56e0\u4e3a\u53d1\u73b01.922\u7684\u4e59\u9178\u7532\u916f\u8d28\u5b50\u4fe1\u53f7\u4e0e\u8840\u7ba1\u7d27\u5f20\u7d20I\u7684\u8d28\u5b50\u4fe1\u53f7\u91cd\u53e0\uff0c\u6240\u4ee5\u6700\u521d\u5236\u5907\u8840\u7ba1\u7d27\u5f20\u7d20\u2160\u7684\u4e0d\u542b\u4e59\u9178\u76d0\u7684\u6837\u54c1\uff0c\u4ee5\u786e\u5b9a\u4e59\u9178\u76d0\u5cf0\u4e0b\u4fe1\u53f7\u7684\u51c6\u786e\u8d28\u5b50\u8ba1\u6570\u3002\u901a\u8fc7\u75280.02mol\/L HCl\u5bf9\u6750\u6599\u8fdb\u884c\u4e24\u6b21\u51b7\u51bb\u5e72\u71e5\u6765\u5b9e\u73b0\u4ece\u8be5\u6837\u54c1\u4e2d\u53bb\u9664\u4e59\u9178\u76d0\u3002\u901a\u8fc7\u8d28\u5b50NMR\u5bf9\u8be5\u6837\u54c1\u7684\u5206\u6790\u8868\u660e\u5176\u4e0d\u542b\u4e59\u9178\u76d0\uff0c\u5e76\u4e14\u5728\u611f\u5174\u8da3\u7684\u5149\u8c31\u533a\u57df\u4e2d\u7684\u8d28\u5b50\u8ba1\u6570\u4e3a3\u3002\u6b64\u540e\uff0c\u5728\u4e0d\u51b7\u51bb\u5e72\u71e5\u7684\u60c5\u51b5\u4e0b\u5206\u6790SRM\u7684\u6837\u54c1\uff0c\u5e76\u4ece\u4e59\u9178\u7532\u916f\u5cf0\u53ca\u5176\u6f5c\u5728\u4fe1\u53f7\u7684\u603b\u79ef\u5206\u4e2d\u51cf\u53bb\u4e09\u4e2a\u8d28\u5b50\u7684\u9002\u5f53\u79ef\u5206\u503c\uff0c\u4ee5\u83b7\u5f97\u4e59\u9178\u5cf0\u7684\u79ef\u5206\u3002\u4e09\u4e2a\u8d28\u5b50\u7684\u79ef\u5206\u7684\u9002\u5f53\u503c\u662f\u901a\u8fc7\u5bf9\u9ad8\u573a\u533a\u57df\u4e2d\u603b\u517129\u4e2a\u8d28\u5b50\u4fe1\u53f7\u7684\u79ef\u5206\u8fdb\u884c\u5e73\u5747\u6765\u786e\u5b9a\u7684\uff0c\u5176\u4e2d\u5305\u62ec\u8bb8\u591a\u6e90\u81ea\u8840\u7ba1\u7d27\u5f20\u7d20I\u7684\u7532\u57fa\u3001\u4e9a\u7532\u57fa\u548c\u6b21\u7532\u57fa\u8d28\u5b50\u7684\u4fe1\u53f7\u3002\u8d28\u5b50\u4fe1\u53f7\u7684\u6570\u5b57\u79ef\u5206\u7ed8\u5236\u5728\u56fe\u8868\u7eb8\u4e0a\uff0c\u5e76\u624b\u52a8\u6d4b\u91cf\u3002\u4f7f\u7528\u4e86\u4ee5\u4e0b\u4eea\u5668\u53c2\u6570\uff1a\u6570\u636e\u96c6\u5927\u5c0f\uff0c16384\u70b9\uff1b4\u00b5s\uff0830\u00b0\u7ffb\u8f6c\u89d2\u5ea6\uff09\uff1b\u9891\u8c31\u5bbd\u5ea6\uff0c4kHz\uff1b\u626b\u63cf\u6b21\u6570\uff0c2000\u6b21\uff1b\u5f1b\u8c6b\u5ef6\u8fdf1.95s\u3002<\/p>\n

NMR\u672a\u68c0\u6d4b\u5230\u9664\u8840\u7ba1\u7d27\u5f20\u7d20I\u548c\u4e59\u9178\u76d0\u4ee5\u5916\u7684\u5176\u4ed6\u7269\u8d28\u3002\u56e0\u6b64\uff0c\u8840\u7ba1\u7d27\u5f20\u7d20I\u7684\u6d53\u5ea6\uff0c\u6309\u5dee\u503c\u8ba1\uff0c\u4e3a93.7\u91cd\u91cf%\u3002<\/p>\n

\u901a\u8fc7\u4ece\u6574\u6279\u8840\u7ba1\u7d27\u5f20\u7d20I\u4e2d\u968f\u673a\u9009\u62e910\u652f\u5b89\u74ff\u6765\u8bc4\u4f30\u704c\u88c5\u5b89\u74ff\u7684\u518d\u73b0\u6027\u3002\u5c06\u6bcf\u652f\u5b89\u74ff\u7684\u5185\u5bb9\u7269\u6eb6\u89e3\u57282 mL\u524d\u8ff0HPLC\u6d17\u8131\u6db2\uff08\u6bd4\u4f8b81:19\uff09\u4e2d\uff0c\u5e76\u5728\u5341\u516b\u70f7\u57fa\u7845\u70f7\u67f1\u4e0a\u5bf9100\u00b5L\u7b49\u5206\u8bd5\u6837\u7684\u8be5\u6eb6\u6db2\u8fdb\u884c\u8272\u8c31\u5206\u6790\u3002\u4f7f\u7528215nm\u7684\u5cf0\u5bf9\u8840\u7ba1\u7d27\u5f20\u7d20I\u8fdb\u884c\u91cd\u590d\u7684HPLC\u6d4b\u91cf\u3002\u5b89\u74ff\u4e4b\u95f4\u7684\u8ba1\u7b97\u53d8\u5f02\u7cfb\u6570\u4e3a2.9%\u3002<\/p>\n

\u4f7f\u7528\u4e94\u4e2a\u968f\u673a\u9009\u62e9\u7684\u5b89\u74ff\u6d4b\u5b9a\u6bcf\u4e2a\u5b89\u74ff\u4e2d\u7684\u603b\u80bd\uff0c\u4e00\u5f0f\u4e24\u4efd\u3002\u5c06\u6bcf\u4e2a\u5b89\u74ff\u7684\u5185\u5bb9\u7269\u6eb6\u89e3\u5728\u79f0\u91cd\u91cf\u4e3a0.1mol\/L\u7684HCl\u4e2d\u3002\u5c06\u8be5\u6eb6\u6db2\u7684\u7b49\u5206\u8bd5\u6837\u8f6c\u79fb\u5230\u79f0\u91cd\u7684\u5c0f\u74f6\u4e2d\uff0c\u5e76\u6d4b\u5b9a\u7b49\u5206\u8bd5\u6837\u7684\u91cd\u91cf\u3002\u7136\u540e\u52a0\u5165\u7b49\u5206\u7684\u82ef\u4e19\u6c28\u9178\u6eb6\u6db2\uff086.2mmol\/L\uff09\u5e76\u79f0\u91cd\u3002\u5c06\u6240\u5f97\u6df7\u5408\u7269\u57286mol\/L HCl\u4e2d\u6c34\u89e3\uff0c\u5e76\u6d4b\u5b9a\u6c28\u57fa\u9178\u7ec4\u6210\u3002<\/p>\n

\u80bd\u7684\u91cf\u7531\u4ee5\u4e0b\u65b9\u7a0b\u8ba1\u7b97\uff1a<\/p>\n

\u5f0f\u4e2d\uff0cX=\u5b89\u74ff\u4e2d\u7684\u80bd\u00b5mol\uff0cM=\u6dfb\u52a0\u7684\u82ef\u4e19\u6c28\u9178\u00b5mol\uff0cLs=\u52a0\u6807\u6837\u54c1\u4e2d\u7684\u4eae\u6c28\u9178\u5cf0\u9ad8\u5ea6\uff0cPst=\u6807\u51c6\u6c28\u57fa\u9178\u6df7\u5408\u7269\u4e2d\u7684\u82ef\u4e19\u6c28\u9178\u5cf0\u9ad8\u5ea6\uff0cLst=\u6807\u51c6\u6c28\u57fa\u9178\u6df7\u5408\u6db2\u4e2d\u7684\u4eae\u6c28\u9178\u5cf0\u503c\u9ad8\u5ea6\uff0cPs=\u52a0\u6807\u6837\u672c\u4e2d\u7684\u82ef\u4e19\u6c28\u9178\u5cf0\u503c\u9ad8\u5ea6\u3002\u6837\u54c1\u7684\u5e73\u5747\u91cd\u91cf\u4e3a526\u00b5g\uff0c\u6807\u51c6\u8bef\u5dee\u4e3a10\u00b5g\u3002\u5b89\u74ff\u4e4b\u95f4\u7684\u8ba1\u7b97\u53d8\u5f02\u7cfb\u6570\u4e3a4.0%\u3002\u5f53\u7cbe\u6c28\u9178\u7684\u5cf0\u9ad8\u53d6\u4ee3\u4eae\u6c28\u9178\u7684\u5cf0\u9ad8\u65f6\uff0c\u83b7\u5f97\u4e86\u7c7b\u4f3c\u7684\u7ed3\u679c\u3002\u8fd9\u4e9b\u7ed3\u679c\u4e0e\u5b89\u74ff\u704c\u88c5\u7814\u7a76\u76f8\u7ed3\u5408\uff0c\u8868\u660e\u5e0c\u671b\u83b7\u5f97\u975e\u5e38\u7cbe\u786e\u6d4b\u91cf\u7684\u4f7f\u7528\u8005\u5fc5\u987b\u5728\u4f7f\u7528\u524d\u79f0\u91cd\u8840\u7ba1\u7d27\u5f20\u7d20I\u3002<\/p>\n

\u901a\u8fc7Benson\u548cHare\u7684\u8272\u8c31\u6cd5\uff08Proc.Natl.Acad.Sci.\uff0cU.S.Vol.72\uff0cp.1391975\uff09\u6d4b\u5b9a9\u4e2a\u968f\u673a\u9009\u62e9\u7684\u6837\u54c1\u7684\u6c28\u57fa\u9178\u7ec4\u6210\u3002\u6c28\u57fa\u9178\u7684\u6469\u5c14\u6bd4\u4e3a\uff1a\u5929\u51ac\u6c28\u9178\uff08Asp\uff091.01\uff0c\u7cbe\u6c28\u9178\uff08Arg\uff090.95\uff0c\u7f2c\u6c28\u9178\uff08Val\uff091.05\uff0c\u916a\u6c28\u9178\uff08Tyr\uff090.97\uff0c\u5f02\u4eae\u6c28\u9178\uff08Ile\uff091.0l\uff0c\u82ef\u4e19\u6c28\u9178\uff08Phe\uff091.05\u3001\u4eae\u6c28\u9178\uff08Leu\uff091.04\u548c\u7ec4\u6c28\u9178\uff08His\uff091.94\u3002\u8be5\u65b9\u6cd5\u4e0d\u5141\u8bb8\u6d4b\u5b9aD-\u6c28\u57fa\u9178\u3002<\/p>\n

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