{"id":103295,"date":"2025-07-14T15:58:14","date_gmt":"2025-07-14T07:58:14","guid":{"rendered":"http:\/\/www.broadbio.com\/?p=103295"},"modified":"2025-07-14T15:58:17","modified_gmt":"2025-07-14T07:58:17","slug":"%e6%8c%87%e7%a4%ba%e5%89%82%e5%88%86%e7%b1%bb","status":"publish","type":"post","link":"http:\/\/www.broadbio.com\/archives\/103295","title":{"rendered":"\u6307\u793a\u5242\u5206\u7c7b"},"content":{"rendered":"\n

\u6307\u793a\u5242\u5206\u7c7b<\/strong><\/strong><\/p>\n\n\n\n

1\u3001\u9178\u78b1\u6307\u793a\u5242\u3002\u6307\u793a\u6eb6\u6db2\u4e2dH+\u6d53\u5ea6\u7684\u53d8\u5316\uff0c\u662f\u4e00\u79cd\u6709\u673a\u5f31\u9178\u6216\u6709\u673a\u5f31\u78b1\uff0c\u5176\u9178\u6027\u548c\u78b1\u6027\u5177\u6709\u4e0d\u540c\u7684\u989c\u8272\u3002\u6307\u793a\u5242\u9178HIn\u5728\u6eb6\u6db2\u4e2d\u7684\u79bb\u89e3\u5e38\u6570Ka=[H+][In-]\/[HIn]\uff0c\u5373\u6eb6\u6db2\u7684\u989c\u8272\u51b3\u5b9a\u4e8e[In-]\/[HIn]\uff0c\u800c[In-]\/[HIn]\u53c8\u51b3\u5b9a\u4e8e[H+]\u3002\u4ee5\u7532\u57fa\u6a59(Ka=10-3.4)\u4e3a\u4f8b\uff0c\u6eb6\u6db2\u7684pH<3.1\u65f6\uff0c\u5448\u9178\u6027\uff0c\u5177\u7ea2\u8272;ph>4.4\u65f6\uff0c\u5448\u78b1\u6027\uff0c\u5177\u9ec4\u8272;\u800c\u5728pH3.1~4.4\uff0c\u5219\u51fa\u73b0\u7ea2\u9ec4\u7684\u6df7\u5408\u8272\u6a59\u8272\uff0c\u79f0\u4e4b\u4e3a\u6307\u793a\u5242\u7684\u53d8\u8272\u8303\u56f4\u3002\u4e0d\u540c\u7684\u9178\u78b1\u6307\u793a\u5242\u6709\u4e0d\u540c\u7684\u53d8\u8272\u8303\u56f4\u3002<\/p>\n\n\n\n

2\u3001\u91d1\u5c5e\u6307\u793a\u5242\u3002\u7edc\u5408\u6ef4\u5b9a\u6cd5\u6240\u7528\u7684\u6307\u793a\u5242\uff0c\u5927\u591a\u662f\u67d3\u6599\uff0c\u5b83\u5728\u4e00\u5b9apH\u4e0b\u80fd\u4e0e\u91d1\u5c5e\u79bb\u5b50\u7edc\u5408\u5448\u73b0\u4e00\u79cd\u4e0e\u6e38\u79bb\u6307\u793a\u5242\u5b8c\u5168\u4e0d\u540c\u7684\u989c\u8272\u800c\u6307\u793a\u7ec8\u70b9\u3002<\/p>\n\n\n\n

3\u3001\u6c27\u5316\u8fd8\u539f\u6307\u793a\u5242\u3002\u4e3a\u6c27\u5316\u5242\u6216\u8fd8\u539f\u5242\uff0c\u5b83\u7684\u6c27\u5316\u5f62\u4e0e\u8fd8\u539f\u5f62\u5177\u6709\u4e0d\u540c\u7684\u989c\u8272\uff0c\u5728\u6ef4\u5b9a\u4e2d\u88ab\u6c27\u5316(\u6216\u8fd8\u539f)\u65f6\uff0c\u5373\u53d8\u8272\uff0c\u6307\u793a\u51fa\u6eb6\u6db2\u7535\u4f4d\u7684\u53d8\u5316\u3002<\/p>\n\n\n\n

4\u3001\u6c89\u6dc0\u6ef4\u5b9a\u6307\u793a\u5242\u3002\u4e3b\u8981\u662fAg+\u4e0e\u5364\u7d20\u79bb\u5b50\u7684\u6ef4\u5b9a\uff0c\u4ee5\u94ec\u9178\u94be\u3001\u94c1\u94f5\u77fe\u6216\u8367\u5149\u9ec4\u4f5c\u6307\u793a\u5242\u3002<\/p>\n\n\n\n

\u5728\u5b9e\u9645\u5de5\u4f5c\u4e2d\uff0c\u8089\u773c\u662f\u96be\u4ee5\u51c6\u786e\u5730\u89c2\u5bdf\u51fa\u6307\u793a\u5242\u53d8\u8272\u70b9\u989c\u8272\u7684\u5fae\u5c0f\u7684\u6539\u53d8\u3002\u4eba\u4eec\u76ee\u6d4b\u9178\u78b1\u6307\u793a\u5242\u4ece\u4e00\u79cd\u989c\u8272\u53d8\u4e3a\u53e6\u4e00\u79cd\u989c\u8272\u7684\u8fc7\u7a0b\uff0c\u53ea\u80fd\u5728\u4e00\u5b9a\u7684pH\u53d8\u5316\u8303\u56f4\u5185\u624d\u80fd\u53d1\u751f\uff0c\u5373\u53ea\u6709\u5f53\u4e00\u79cd\u989c\u8272\u76f8\u5f53\u4e8e\u53e6\u4e00\u79cd\u989c\u8272\u6d53\u5ea6\u7684\u5341\u500d\u65f6\u624d\u80fd\u52c9\u5f3a\u8fa8\u8ba4\u5176\u989c\u8272\u7684\u53d8\u5316\u3002\u5728\u8fd9\u79cd\u989c\u8272\u53d8\u5316\u7684\u540c\u65f6\uff0c\u4ecb\u8d28\u6c22\u706d\u83cc\u751f\u7269\u6307\u793a\u5242\u7684pH\u503c\u5219\u7531\u4e00\u4e2a\u503c\u53d8\u5230\u53e6\u4e00\u4e2a\u503c\u3002<\/p>\n\n\n\n

\u5f53\u6eb6\u6db2\u7684pH\u503c\u5927\u4e8epKHIn\u65f6, [In-]\u5c06\u5927\u4e8e[HIn]\u4e14\u5f53[In-]\/[HIn]=10\u65f6\uff0c\u6eb6\u6db2\u5c06\u5b8c\u5168\u5448\u73b0\u78b1\u8272\u6210\u5206\u7684\u989c\u8272\uff0c\u800c\u9178\u8272\u88ab\u906e\u76d6\u4e86\uff0c\u8fd9\u65f6\u6eb6\u6db2\u7684 pH=pKHIn+1\u3002\u540c\u7406\uff0c\u5f53\u6eb6\u6db2\u7684pH\u503c\u5c0f\u4e8epKHIn\u65f6, [In-]\u5c06\u5c0f\u4e8e[HIn]\u4e14\u5f53[In-]\/[HIn]=1\/10\u65f6\uff0c\u6eb6\u6db2\u5c06\u5b8c\u5168\u5448\u73b0\u9178\u8272\u6210\u5206\u7684\u989c\u8272\uff0c\u800c\u78b1\u8272\u88ab\u906e\u76d6\u4e86\uff0c\u8fd9\u65f6\u6eb6\u6db2\u7684 pH=pKHIn-1\u3002<\/p>\n\n\n\n

\u53ef\u89c1\u6eb6\u6db2\u7684\u989c\u8272\u662f\u5728\u4ecepH=pKHIn-1\u5230 pH=pKHIn+1\u7684\u8303\u56f4\u5185\u53d8\u5316\u7684\uff0c\u8fd9\u4e2a\u8303\u56f4\u79f0\u4e3a\u6307\u793a\u5242\u7684\u53d8\u8272\u8303\u56f4\u5373\u53d8\u8272\u57df\u3002\u5728\u53d8\u8272\u8303\u56f4\u5185\uff0c\u5f53\u6eb6\u6db2\u7684pH\u503c\u6539\u53d8\u65f6\uff0c\u78b1\u8272\u6210\u5206\u548c\u9178\u8272\u6210\u5206\u7684\u6bd4\u503c\u968f\u4e4b\u6539\u53d8\uff0c\u6307\u793a\u5242\u7684\u989c\u8272\u4e5f\u53d1\u751f\u6539\u53d8\u3002\u8d85\u51fa\u8fd9\u4e2a\u8303\u56f4\uff0c\u5982pH\u2265pKHIn+1\u65f6\uff0c\u770b\u5230\u7684\u53ea\u662f\u78b1\u8272;\u800c\u5728pH\u2264pKHIn-1\u65f6\uff0c\u5219\u770b\u5230\u7684\u53ea\u662f\u9178\u8272\u3002\u56e0\u6b64\u6307\u793a\u5242\u7684\u53d8\u8272\u8303\u56f4\u7ea62\u4e2apH\u5355\u4f4d\u3002\u7531\u4e8e\u4eba\u7684\u89c6\u89c9\u5bf9\u5404\u79cd\u989c\u8272\u7684\u654f\u611f\u7a0b\u5ea6\u4e0d\u540c\uff0c\u52a0\u4e0a\u5728\u53d8\u8272\u57df\u5185\u6307\u793a\u5242\u5448\u73b0\u6df7\u5408\u8272\uff0c\u4e24\u79cd\u989c\u8272\u4e92\u76f8\u5f71\u54cd\u89c2\u5bdf\uff0c\u6240\u4ee5\u5b9e\u9645\u89c2\u5bdf\u7ed3\u679c\u4e0e\u7406\u8bba\u503c\u6709\u5dee\u522b\uff0c\u5927\u591a\u6570\u6307\u793a\u5242\u7684\u53d8\u8272\u8303\u56f4\u5c0f\u4e8e2\u4e2aPH\u5355\u4f4d\u3002<\/p>\n\n\n\n

\u514d\u8d23\u58f0\u660e\uff1a<\/strong><\/p>\n\n\n\n

\u672c\u6587\u4fe1\u606f\u90e8\u5206\u5185\u5bb9\u6765\u81ea\u7f51\u7edc\u6587\u7ae0\uff0c\u4e0d\u4fdd\u8bc1\u6240\u6709\u4fe1\u606f\u3001\u6587\u672c\u3001\u56fe\u5f62\u3001\u94fe\u63a5\u53ca\u5176\u4ed6\u9879\u76ee\u7684\u7edd\u5bf9\u51c6\u786e\u6027\u548c\u5b8c\u6574\u6027\uff0c\u6545\u4ec5\u4f9b\u8bbf\u95ee\u8005\u53c2\u7167\u4f7f\u7528\u3002\u5982\u60a8\uff08\u5355\u4f4d\u6216\u4e2a\u4eba\uff09\u8ba4\u4e3a\u672c\u6587\u67d0\u90e8\u5206\u5185\u5bb9\u6709\u4fb5\u6743\u5acc\u7591\uff0c\u656c\u8bf7\u7acb\u5373\u901a\u77e5\u6211\u4eec\uff0c\u6211\u4eec\u5c06\u5728\u7b2c\u4e00\u65f6\u95f4\u4e88\u4ee5\u66f4\u6b63\u6216\u5220\u9664\u3002\u4ee5\u4e0a\u58f0\u660e\u4e4b\u89e3\u91ca\u6743\u5f52\u672c\u516c\u53f8\u6240\u6709\u3002\u6cd5\u5f8b\u4e0a\u6709\u76f8\u5173\u89e3\u91ca\u7684\uff0c\u4ee5\u4e2d\u56fd\u6cd5\u5f8b\u4e4b\u89e3\u91ca\u4e3a\u57fa\u51c6\u3002\u5982\u6709\u4e89\u8bae\u9650\u5728\u6211\u65b9\u6240\u5728\u5730\u53f8\u6cd5\u90e8\u95e8\u89e3\u51b3\u3002\u8c22\u8c22\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u6307\u793a\u5242\u5206\u7c7b 1\u3001\u9178\u78b1\u6307\u793a\u5242\u3002\u6307\u793a\u6eb6\u6db2\u4e2dH+\u6d53\u5ea6\u7684\u53d8\u5316\uff0c\u662f\u4e00\u79cd\u6709\u673a\u5f31\u9178\u6216\u6709\u673a\u5f31\u78b1\uff0c\u5176\u9178\u6027\u548c\u78b1\u6027\u5177\u6709\u4e0d\u540c\u7684\u989c\u8272\u3002\u6307 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[92],"tags":[],"_links":{"self":[{"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/posts\/103295"}],"collection":[{"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/comments?post=103295"}],"version-history":[{"count":1,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/posts\/103295\/revisions"}],"predecessor-version":[{"id":103296,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/posts\/103295\/revisions\/103296"}],"wp:attachment":[{"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/media?parent=103295"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/categories?post=103295"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.broadbio.com\/wp-json\/wp\/v2\/tags?post=103295"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}